| Splinter/Hun | Ray Maddox |
| Leonardo | Leroy Diaz |
| Michelangelo | Andrew Skinner |
| Donatello | Leonel Rojas |
| Raphael/SCX/Superious | Brian Hoskins |
| Karai | Farrah Hollabaugh |
| Alice | Michelle Caputo |
| April O'Neal Jones | Alexandra Becker |
| Casey Jones | Tim Downes |
| Benjamin Jones | Kyle Drake |
| Usagi | David Gilmore |
| Ken | Timmy Mucci |
| Brickman | Garrett Robinson |
| Henchmen/Announcer | Sean Something |
| Experiment 842/Bani | Remi Hoberman |
| Shredder | Nick Rivera |
| Samuel | Damion Lopez |
| Irma | Olivia Kharel |
| Ichika | Maria Mucaria |
| TMNT Decennium Creator | Leroy Diaz |
| Opening Theme | Jacob Lizotte |
| Ending Theme: Rose Blood | Head Underwater |
| Audio Editor 1 | Leroy Diaz |
| Audio Editor 2 | That Guy |
| Music Composer | Mark Dobrowolski |